Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7587349 | Food Chemistry | 2017 | 8 Pages |
Abstract
Sotolon is widely associated with the quality of fortified aged wines, and has also been linked to premature oxidative aging (premox). Here we developed a single, fast and environmental-friendly microextraction by packed sorbent ultra-high pressure liquid chromatography analysis (MEPS/UHPLC-PDA) for sotolon quantification in different wines. The best extraction conditions (loading three times 250 μL samples through the MEPSPEP sorbent and elution with 100 μL of 50% MeOH) were combined with a fast UHPLC separation (5 min separation using acidified 10% MeOH isocratic flow in a CORTECS C18 column) to allow unparalleled minimum sample and solvents volumes usage. The validated methodology showed good linearity (r2 > 0.993) and precision (<5.6%); high recovery (>81%) and detection limits (0.45-2.51 μg/L) far below sotolon odor threshold for any type of wine. The methodology was successfully applied to selected white table and Madeira wines, encompassing therefore a wide range of alcohol and sugar contents. Furthermore, as far we may know, this is the first time a single methodology can be used to assess both wine aging or premox according to the type of wine.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jorge Freitas, Rosa Perestrelo, Rubina Cassaca, Mariangie Castillo, Mariana Santos, Jorge Pereira, José S. Câmara,