Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7588770 | Food Chemistry | 2016 | 6 Pages |
Abstract
6-Benzylaminopurine (6-BA), a cytokinin plant growth regulator, has been banned for use in bean sprout production in China. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with a specific monoclonal antibody (mAb 3E5). The assay showed a half-maximum inhibition concentration (IC50) and detection range of 18.9Â ng/mL and 3.6-106Â ng/mL, respectively. Recoveries of 6-BA spiked in home cultured bean sprout samples averaged from 75% to 89% with a correlation coefficient (R2) of 0.998 between the results determined by icELISA and those by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS). LC-ESI-MS showed that 6-BA had been partially metabolized to 6-benzylaminopurine riboside (6-BAR) in the positive samples. The content of 6-BA determined by icELISA was about 5-70 times higher than that of LC-ESI-MS because mAb 3E5 had 315% cross-reactivity with 6-BAR. Such icELISA being ultra-sensitive to 6-BAR would allow quick monitoring of 6-BA by detecting 6-BAR as a potential biomarker.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wei Zhang, Lishan He, Rui Zhang, Suqin Guo, Huanfang Yue, Xiangxue Ning, Guiyu Tan, Qing X. Li, Baomin Wang,