Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7589645 | Food Chemistry | 2016 | 23 Pages |
Abstract
A simple, rapid and precise HPLC assay was developed for the well-known anti-cancer isothiocyanates-sulforaphene (SE) and sulforaphane (SF). The analytical system comprised RP-C18 column with isocratic 5% THF-95% water. High resolution was obtained (and eluted) of two distinct HPLC peaks of similar structures SE and SF analytes (at 23.01 ± 0.02 and 25.65 ± 0.03 min, respectively). The respective LOD vs. LOQ for SE and SF was 0.34 and 0.36 μg/ml vs. 1.02 and 1.08 μg/ml. This assay had the best linearity and accuracy. The recoveries were in the range of 96.83-101.17%. SF and SE were quantified in the pod of Raphanus sativus L. var. caudatus Alef extracts (2253.05 ± 246.18 and 111.94 ± 16.49 μg/g in the crude extract, respectively), while only SE was detected in the stem (1105.14 ± 243.10 μg/g crude extract), as SF was lower than the detection limit. The validated method thus minimized and expedited simultaneous SE and SF analysis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sarita Sangthong, Natthida Weerapreeyakul,