Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7589716 | Food Chemistry | 2016 | 8 Pages |
Abstract
β-Lactoglobulin (β-LG) was conjugated with monomethoxy polyethylene glycol-succinimidyl carbonates (mPEG-SC, 20 kDa) to investigate the relationship between the antigenicity and conformational changes of β-LG. The effect of molar ratio of protein to mPEG-SC (1:3-1:6), pH (6-8) and time (4-24 h) on the antigenicity of β-LG was investigated. The lowest antigenicity of β-LG was observed at the molar ratio of 1:3, pH 7.0, and reaction time for 8 h, which was 70% lower than that of control β-LG. At the optimal modification conditions, it was indicated that two fractions obtained after purification showed the tense and single band on the SDS-PAGE at the position of approximate 78 kDa and 58 kDa, which corresponded to the tri- and di-PEGylated conjugate, respectively. As conjugated number of mPEG-SC with β-LG increased, the quenching of fluorescence and the content of β-strands were increased gradually, which may contribute to the decrease of antigenicity from two aspects.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Junzhen Zhong, Xiaofei Cai, Chengmei Liu, Wei Liu, Yujia Xu, Shunjing Luo,