Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7590216 | Food Chemistry | 2016 | 6 Pages |
Abstract
Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity (r ⩾ 0.9994) and afforded low limits of detection ranging from 1.55 to 10.17 μg Lâ1 for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% (n = 6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chengzhu Ni, Binhe Zhu, Nani Wang, Muhua Wang, Suqing Chen, Jiajie Zhang, Yan Zhu,