Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7590404 | Food Chemistry | 2016 | 9 Pages |
Abstract
Pressurised water extraction (PWE) of polysaccharides from blackcurrant fruits was investigated using a response surface methodology (RSM). The optimal conditions for PWE were: time 51 min, pressure 1.6 MPa, and temperature 52 °C. Under these conditions, the experimental yield of Ribes nigrum L. polysaccharides (RNLP) was 11.68 ± 0.12%, which closely agreed with the predicted value (11.77%). After preliminary purification with D4006 macroporous resin, RNLP I was obtained and its chemical characterisation was undertaken by GC, HPLC, and IR spectroscopy. RNLP I was composed of rhamnose, arabinose, xylose, mannose, galactose, and glucose with a molar ratio of 2.89:14.82:1.02:1.00:2.53:6.39 and its molecular weight was 1.49 Ã 104 kDa. The antioxidant activity of RNLP I was evaluated by free radical scavenging assays and a reducing power assay in vitro. RNLP I showed strong DPPH and superoxide radical scavenging activities and reducing power.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yaqin Xu, Fei Cai, Zeyuan Yu, Ling Zhang, Xingguo Li, Yu Yang, Gaijie Liu,