Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7590886 | Food Chemistry | 2016 | 12 Pages |
Abstract
The Chilean raspberry Rubus geoides Sm. (Rosaceae) is a native species occurring in the Patagonia. Five R. geoides samples were assessed for phenolic content and composition, antioxidant activity, effect on total reduced glutathione (GSH) synthesis and protective effect against H2O2 and methylglyoxal (MGO)-induced stress in epithelial gastric AGS cells. The HPLC-DAD/ESI-MS profiles allowed the tentative identification of 39 phenolics including flavonol glycosides and tannins. R. geoides presented higher total phenolic and flavonoid content than Rubus idaeus. Two out of the five phenolic enriched R. geoides extracts (PEEs) exhibited better antioxidant activity than R. idaeus in the DPPH, FRAP and TEAC assays. A significant cytoprotective activity was observed when AGS cells were pre-incubated with extracts and subsequently challenged with H2O2 or MGO. Treatment with the PEEs increased the intracellular GSH content. R. geoides fruit extracts may induce the activation of intracellular protection mechanisms against oxidative and dicarbonyl-induced stress.
Keywords
FRAPHexahydroxydiphenoylHHDPIrhN-acetyl-l-cysteineTPTZGAEDADTEACAGSNACGSHABTSDPPHESIFBSMTTROSPEEgallic acid equivalentsIsorhamnetindiode array detectionOxidative stressRosaceaefetal bovine serumAGS cellsMass spectrometryTrolox equivalent antioxidant capacityMethanolMethylglyoxaltrolox equivalentquercetin equivalentsMgOMeOHKaempferolferric reducing antioxidant powerreduced glutathionehigh performance liquid chromatographyHPLCTotal flavonoidsTotal phenolicsQuercetinGlutathioneReactive oxygen specieselectrospray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Felipe Jiménez-Aspee, Cristina Theoduloz, Felipe Ávila, Samanta Thomas-Valdés, Claudia Mardones, Dietrich von Baer, Guillermo Schmeda-Hirschmann,