Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7590913 | Food Chemistry | 2016 | 8 Pages |
Abstract
Polyoxyethylene lauryl ether (POELE10)-NaH2PO4 aqueous two-phase extraction system (ATPES) is coupled with HPLC to analyze chloramphenicol (CAP) in aquatic product. Response surface methodology (RSM) was adopted in the multi-factor experiment to determine the optimized conditions. The extraction efficiency of CAP (E%) is up to 99.42% under the optimal conditions, namely, the concentration of NaH2PO4, the concentration of POELE10, pH and temperature were 0.186 g·mLâ1, 0.033 g·mLâ1, 3.8 and 25 °C respectively. The optimal value of enrichment factor of CAP (F) was 22.56 when the concentration of NaH2PO4 was 0.192 g·mLâ1, the concentration of POELE10 was 0.024 g/ml, pH was 4.2 and temperature was 30 °C. The limit of detection (LOD) and limit of quantification (LOQ) of this method are 0.8 μg·kgâ1 and 1 μg·kgâ1, which meet the needs of determining trace or ultratrace CAP in food. The E% and F of this technique are much better than other extraction methods.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yang Lu, Hui Yao, Chuang Li, Juan Han, Zhenjiang Tan, Yongsheng Yan,