Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7592141 | Food Chemistry | 2015 | 6 Pages |
Abstract
The copper-soap method, which is based on the absorbance of a fatty acid-copper complex at 715Â nm, is a widely used colorimetric assay to determine the lipase activity in reversed micellar system. However, the absorbance of the bis(2-ethylhexyl) sodium sulfosuccinate (AOT)-copper complex prevents the use of an AOT/isooctane reversed micellar system. An extraction step was added to the original procedure to remove AOT and eliminate interference from the AOT-copper complex. Among the solvents tested, acetonitrile was determined to be the most suitable because it allows for the generation of a reproducible calibration curve with oleic acid that is independent of the AOT concentrations. Based on the validation data, the modified method, which does not experience interference from the AOT-copper complex, could be a useful method with enhanced accuracy and reproducibility for the lipase assay.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chang Woo Kwon, Kyung-Min Park, Seung Jun Choi, Pahn-Shick Chang,