Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7592661 | Food Chemistry | 2015 | 9 Pages |
Abstract
In this work, we propose a new quick and accurate analytical method by UHPLC-QqQ-MS/MS which is able to identify free phytoprostanes in olive and refined sunflower oils. The recovery provided high extraction efficiencies ranging from 102.90% to 140.64% using Strata-XAW cartridge. The intra-day and inter-day variations for all target compounds ranged from 2.24% to 13.64% and 0.01% to 13.69%, respectively, and the accuracies for these parameters varied from 80.33% to 119.64% and from 80.34% to 119.90%, respectively. Results obtained reflect that refined sunflower presented more series of phytoprostanes and a 20 and 8-fold higher quantity than two types of olive oil: Extra virgin olive oil and olive oil, (containing half virgin extra olive oil and half refined olive oil). The manufacture process could be the key for the different phytoprostane production since most of the plant oils are subjected to a refining treatment.
Keywords
ALASPEMRMRSDUHPLCPhytoPMUFALOQGC–MSROSSolid-phase extractionα-linolenic acidArachidonic acidPolyunsaturated fatty acidmonounsaturated fatty acidPUFArelative standard deviationOxidative stressLOD یا Limit of detectionSunflower oilOlive oilMass spectrometryphytoprostanePhytoprostaneslimit of quantificationlimit of detectionICHmultiple reaction monitoringprostaglandinhigh performance liquid chromatographyHPLCgas chromatography coupled to mass spectrometryInternational Conference on HarmonizationReactive oxygen species
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jacinta Collado-González, Sonia Medina, Thierry Durand, Alexandre Guy, Jean-Marie Galano, Arturo Torrecillas, Federico Ferreres, Angel Gil-Izquierdo,