Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7593297 | Food Chemistry | 2015 | 6 Pages |
Abstract
Protein phosphorylation changes of the sarcoplasmic and myofibrillar proteins in beef longissimus muscle in response to electrical stimulation (ES) was investigated. Sarcoplasmic and myofibrillar proteins purified from muscle samples taken at 0, 3 and 10Â h after ES were separated on SDS-PAGE and stained with phosphorous and protein specific stains. There was a significant effect of ES on phosphorylation of total sarcoplasmic and myofibrillar proteins (PÂ <Â 0.05). However, although there an instant effect of ES on the phosphorylation level of the myofibrillar proteins, the ES effect on the sarcoplasmic proteins (PÂ <Â 0.05) was first observed after 3Â h. Several protein bands were analyzed by LC-MS/MS, revealing that the major glycolytic proteins, including glycogen debranching enzyme, glycogen phosphorylase and 6-phosphofructokinase probably are affected by ES together with different heat shock proteins. This work gives an insight into the regulation of the glycolytic enzymes and muscle contraction on application of electrical stimulation.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chunbao Li, Guanghong Zhou, Xinglian Xu, Kerstin Lundström, Anders Karlsson, René Lametsch,