Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7593907 | Food Chemistry | 2015 | 9 Pages |
Abstract
Dieckol was previously reported to exhibit antioxidant and anticancer activities in vitro studies. In this study, we characterised the mechanism underlying the dieckol-mediated expression of antioxidant and detoxifying enzymes. Dieckol suppressed the production of intracellular reactive oxygen species in the presence or absence of H2O2 and increased glutathione level in HepG2 cells. Dieckol enhanced the activities of antioxidant enzymes, and the expression of detoxifying enzymes including heme oxygenase-1 (HO-1), NAD(P)H:quinine oxidoreductase 1 (NQO1), and glutathione S-transferase (GST) in HepG2 cells. Enhanced expression of antioxidant and detoxifying enzymes by dieckol was presumed to be the activation of the nuclear factor erythroid-derived 2-like 2 (Nrf2) demonstrated by its nuclear translocation and transcriptional activity via activation of mitogen-activated protein kinases in HepG2 cells. Furthermore, we demonstrated dieckol induced the expression of HO-1 in mouse liver. These results demonstrate that the dieckol-mediated cytoprotection in HepG2 cells is mediated through a ROS-independent up-regulation of antioxidant and detoxifying enzymes via Nrf2 activation as well as its intrinsic antioxidant activity, suggesting that dieckol may be used as a natural cytoprotective agent.
Keywords
ALTFBSDCF-DAECLDPPHMTSDAPIGSHGST2′,7′-dichlorofluorescein diacetate2,2-diphenyl-1-picrylhydrazyl4′,6-diamidino-2-phenylindoleBSAkeap1DMSOMAPKsASTAspartate aminotransferaseAlanine aminotransferasebovine serum albuminenhanced chemiluminescenceminimum essential mediumDimethyl sulfoxidefetal bovine serumantioxidant response elementMEMAREheme oxygenaseKelch-like ECH-associated protein 1mitogen-activated protein kinasesGlutathioneglutathione S-transferase
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Min-Sup Lee, Bonggi Lee, Kyoung-Eun Park, Tadanobu Utsuki, Taisun Shin, Chul Woong Oh, Hyeung-Rak Kim,