Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7594160 | Food Chemistry | 2015 | 7 Pages |
Abstract
A novel method was developed based on capillary electrophoresis with indirect laser-induced fluorescence detection for the determination of alditols including xylitol, sorbitol, arabinitol and mannitol. The baseline separation was achieved with a capillary with 30 μm inner diameter and running buffer (pH 9.6) composed of 40 mM sodium tetraborate, 5% (v/v) methanol, 10â5 M sodium fluorescein as background fluorescence. Calibration curves (0.2-2.0 mg mLâ1) showed good linear correlations (r > 0.999). The detection limits of the alditols were in the range of 19.0-24.4 μg mLâ1. The intra-day and inter-day RSDs for migration times and peak areas were less than 1.6% and 4.3%, respectively. The average recoveries were in the range of 99.6-105.5%. The developed method was successfully applied to determination of alditols in different real samples, and the results were compared with those of capillary electrophoresis with indirect ultraviolet detection method.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yao Xiao, Yunfang Li, Jinjin Ying, Yu Tian, Yuxiu Xiao, Zhinan Mei,