Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7594858 | Food Chemistry | 2015 | 8 Pages |
Abstract
In this study, we developed an electrochemically-amplified, stem-loop DNA biosensor to detect the peanut allergen Ara h1. Specifically, we electrodeposited a multilayer graphene-gold nanocomposite onto a glassy carbon electrode and then immobilised a thiolated hairpin DNA-biotin probe onto the modified electrode surface. The multilayer graphene-gold composite has good dispersion ability, and can amplify the electrochemical signal due to its high electron-transfer efficiency. The probe was switched to an “off” state in the presence of target DNA. The prepared biosensor demonstrated a linear response ranging from 10â16 to 10â13Â M, with an ultrasensitive detection limit of 0.041Â fM. Moreover, the biosensor showed excellent selectivity, as well as the ability to discriminate between a complementary target and a one-base mismatch or non-complementary sequence. Results show that this prepared DNA biosensor can be successfully used to detect the peanut allergen Ara h1 in a peanut milk beverage. Findings can be applied to the prevention of allergic reactions, thus improving human health and safety.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiulan Sun, Min Jia, Lu Guan, Jian Ji, Yinzhi Zhang, Lili Tang, Zaijun Li,