Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7595119 | Food Chemistry | 2015 | 7 Pages |
Abstract
Soy isoflavones are known as major bioactive compounds in soybean (Glycine max), which is an indispensable food. Despite their utility, the consumption of isoflavones has recently been limited because they exhibit oestrogenic and topoisomerase II inhibitory effects. To assess their intake limitation, accurate, sensitive, and effective quantitative analyses are necessary. In this study, we produced the monoclonal antibody (MAb) against daidzin (DZ) and applied it to an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the simultaneous determination of DZ and genistin (GEN), which are known as two major soy isoflavone glycosides in soy products. Using the DZ-MAb, we developed a sensitive icELISA method, where the limit of detection for DZ and GEN was 1.95Â ng/ml. Several validation analyses revealed that the icELISA is sufficiently accurate and sensitive to be used to assess the overconsumption of soy isoflavones, which would lead to the safe dietary intake of soy products.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Seiichi Sakamoto, Gorawit Yusakul, Benyakan Pongkitwitoon, Madan Kumar Paudel, Hiroyuki Tanaka, Satoshi Morimoto,