Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7595533 | Food Chemistry | 2015 | 6 Pages |
Abstract
Resveratrol oligomers are biologically active polyphenols found in wine. No information about the bioavailability of these polyphenols is available. In order to discover if the resveratrol oligomers can pass the intestinal barrier, transport of the dimer ε-viniferin and the tetramer hopeaphenol was studied in the Caco-2 transwell system. A flux through the cell monolayer could neither be observed for ε-viniferin nor for hopeaphenol (apparent permeability coefficient (Papp) < 1 Ã 10â6 cm sâ1). In contrast, resveratrol showed a Papp of 11.9 Ã 10â6 cm sâ1. Nevertheless, about 16-30% of the oligomers were found in the lysed cellular fraction. This leads to the conclusion that the intestinal absorption rate of the two resveratrol oligomers, ε-viniferin and hopeaphenol, is low and negligible when compared to resveratrol. Therefore, it is unlikely that the oligomers could elicit a systemic biological effect after dietary intake. However, the compounds may act locally on the intestinal epithelium.
Keywords
DMEMLC–UVTRISn.d.TEERGUSSPEFBSDulbecco’s modified Eagle mediumε-ViniferinAutomated sample preparationSolid phase extractionstandard deviationTris(hydroxymethyl)aminomethanenot detectedIntestinal absorptionResveratrolfetal bovine serumapparent permeability coefficientlucifer yellowTrans-epithelial electrical resistancePappGlucuronidase
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ina Willenberg, Maria Michael, Jasmin Wonik, Laura C. Bartel, Michael T. Empl, Nils Helge Schebb,