Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7596044 | Food Chemistry | 2015 | 7 Pages |
Abstract
Taking into account the substantial potential of raspberry processing by-products, pomace extracts from two raspberry cultivars, Meeker and Willamette, were investigated. Total phenolic, flavonoid and anthocyanin contents were determined. Willamette pomace extract (EC50 = 0.042 mg/ml) demonstrated stronger 2,2-diphenyl-1-picrylhydrazyl DPPH radical-scavenging activity than did Meeker pomace extract (EC50 = 0.072 mg/ml). The most pronounced cell growth inhibition effect was obtained in the breast adenocarcinoma cell line, reaching EC50 values of 34.8 and 60.3 μg/ml for Willamette and Meeker extracts, respectively. Both extracts demonstrated favourable non-tumor/tumor cell growth ratios and potently increased the apoptosis/necrosis ratio in breast adenocarcinoma and cervix carcinoma cells. In reference and wild bacterial strains, minimal inhibitory concentrations (MIC) were achieved in a concentration range from 0.29 to 0.59 mg/ml, and minimal bactericidal concentrations (MBC) in a range from 0.39 to 0.78 mg/ml. The results indicate significant antioxidant, antiproliferative, proapoptotic and antibacterial activities of raspberry pomace and favour its use as a functional food ingredient.
Keywords
DMEMFCSTphDulbecco’s modified eagles mediumMBCHT-29GAEMRC-5EC50TACMCF7MICSulphorhodamine BSRBDPPHTFD2,2-diphenyl-1-picrylhydrazylDMSObreast adenocarcinomaColon adenocarcinomaEDTAethylenediamine tetraacetic acidgallic acid equivalentsMinimal bactericidal concentrationMinimal inhibitory concentrationApoptosisdimethyl sulphoxiderutin equivalentshuman fetal lungfetal calf serumFree radical-scavenging activityAntibacterial activityAntitumor activityScavenging activityTotal phenolic contenttotal anthocyanin contentTotal flavonoid contenthalf maximal effective concentrationHela
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dragana D. ÄetojeviÄ-Simin, Aleksandra S. VeliÄanski, Dragoljub D. CvetkoviÄ, SiniÅ¡a L. Markov, Gordana S. ÄetkoviÄ, Vesna T. Tumbas Å aponjac, Jelena J. VuliÄ, Jasna M. ÄanadanoviÄ-Brunet, Sonja M. Djilas,