Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7596551 | Food Chemistry | 2015 | 10 Pages |
Abstract
Natural sources of triacylglycerols containing Ï-3 fatty acids are of particular interest due to their protective role against several human diseases. However, as it has been well ascertained, the position of the Ï-3 fatty acid on the triacylglycerol backbone influences how digestion occurs. In particular, occurrence at the sn-2 position allows optimal intestinal absorption conditions. The analytical protocol for regioisomer characterisation of fatty acids in a triacylglycerol usually requires the use of stereospecific lipases before instrumental identification. In this paper, we propose a more direct instrumental determination of triacylglycerol composition along with sn-2 positional identification of the fatty acids constituents by Liquid Chromatography-High Resolution Mass Spectrometry. Different intensities of product signals obtained in MS2 and MS3 experiments were used to define an interpretative scheme able to rationalise the stereochemistry of the TAGs. Marine matrices like tuna and algae oils have been studied in detail, their triacylglycerols identified and sn-2 positional arrangement of fatty acid constituents assessed.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Claudio Baiocchi, Claudio Medana, Federica Dal Bello, Valeria Giancotti, Riccardo Aigotti, Daniela Gastaldi,