Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7597499 | Food Chemistry | 2014 | 8 Pages |
Abstract
We here report on the use of water as a 'green' extraction solvent for the isolation of isoflavones from okara, a by-product of soymilk production. At a low liquid-to-solid ratio of 20 to 1 and 20 °C, 47% of the isoflavones that can be extracted with 70% aqueous ethanol were extracted. The malonyl-glucosides were fully recovered with a ratio of 20 to 1, while β-glucosides were recovered with an increased liquid-to-solid ratio of 40 to 1. The extraction of aglycones was better at higher ratios, but leveled off before reaching a 100% yield. Temperature hardly affected the total amount of isoflavones. At a 20 to 1 ratio, 20 °C, and pH 10, there was no significant difference (p > 0.05) between isoflavone extraction in water and in 70% aqueous ethanol. The results suggest that water may be used as a green alternative for separation of isoflavones from okara.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lena Jankowiak, Nikolaos Kantzas, Remko Boom, Atze Jan van der Goot,