Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7598132 | Food Chemistry | 2014 | 5 Pages |
Abstract
The aim of this work was to develop a multivariate method for the rapid determination of caffeine and Class IV caramel in cola-type soft drinks and of caffeine, Class III caramel and riboflavin in energy drinks using synchronous fluorescence spectra. The synchronous fluorescence spectra were recorded at constant wavelength difference 90 nm from 200 to 500 nm. Reference values of analyte concentrations by high performance liquid chromatography (HPLC) with fluorescence detection combined with the standard addition method were used to create the partial least squares (PLS) models. High coefficients of determination (>0.99) were obtained in 0.2-4.2, 0.25-5.25, 0.4-10.0 and 0.007-0.054 mg Lâ1 range for caffeine, Class III caramel, Class IV caramel and riboflavin, respectively. The PLS models were used to determine the concentration of analytes in different drink samples. The method provided comparable results with those found using the HPLC method.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
L'udovÃt Žiak, Pavel Májek, KatarÃna HroboÅová, FrantiÅ¡ek Äacho, Jana Sádecká,