Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7598516 | Food Chemistry | 2014 | 7 Pages |
Abstract
Some methodological aspects of an on-line combination of capillary zone electrophoresis with mass spectrometric detection (CZE-QqQ-MS) were studied in this work as well as the possibilities of using this combination for analysis of the high-molecular mass compounds present in multi-component matrices. All experiments using an on-line combination of capillary electrophoresis with mass spectrometric detection were carried out in cationic mode in covalently-coated capillary. The optimised electrolyte system consisted of 100Â mmol/L formic acid. Prior to the CZE-QqQ-MS analysis, an extraction of lysozyme from cheese samples using 1Â mol/L of acetic acid was performed. The LOD was 3.6Â mg lysozyme per kg and the LOQ was 10.9Â mg lysozyme per kg. The concentration range of the lysozyme determined in four cheese samples analysed in this work was from 0.5 to 3.3Â g of lysozyme per kg. The values of the relative standard deviations thus obtained were from 4.6% to 9.3% depending on the cheese sample.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Monika Kondeková, VÃtÄzslav Maier, PavlÃna Ginterová, Jozef Marák, Juraj Å evÄÃk,