Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7599018 | Food Chemistry | 2014 | 8 Pages |
Abstract
Polysaccharides from Musa sapientum (MSP) were isolated and purified by gel filtration chromatography. Elemental analysis, scanning electron microscopy (SEM), X-ray diffraction crystallography (XRD), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR) and NMR techniques were used to characterize the polysaccharide. Elemental analysis of the polysaccharide indicated that the contents of carbon, hydrogen and nitrogen to be 27.31, 5.07 and 0.62 (w/w%) respectively. SEM analysis suggested that the polysaccharide had irregular particle size, mostly seen as aggregates, and fibrous in nature. The samples had peaks at approximately 25°, 32°, 40° and 43° 2θ degrees in the XRD pattern, which indicated both crystalline and amorphous structure. The major functional groups identified from FT-IR spectrum includes 1631.38 cmâ1 (-COO-), 1377 cmâ1 (-COO-) and 3193.93 cmâ1 (-OH). Analytical data on MSP indicated that the major neutral sugars were α-d-glucose, β-d-glucose, rhamnose and d-Glucuronic acid. The experimental work provides enough evidence to exploit this neutral biopolymer in food and pharmaceutical industry.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dash Suvakanta, Murthy Padala Narsimha, Deb Pulak, Chakraborty Joshabir, Das Biswajit,