Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7599086 | Food Chemistry | 2014 | 8 Pages |
Abstract
Extensive proteolysis takes place during the processing of dry-cured ham due to the action of muscle peptidases. The aim of this work was to study the degradation of LIM domain binding protein 3 (LDB3), which is located at the Z-lines of the sarcomere, at different times during the Spanish dry-cured ham processing (2, 3.5, 5, 6.5, and 9Â months). A total of 107 peptides have been identified by mass spectrometry, most of them generated from the first region of the protein sequence (position 1-90) providing evidence for the complexity and variability of proteolytic reactions throughout the whole process of dry-curing. Methionine oxidation has been observed in several peptides by the end of the process. The potential of some of the identified peptides to be used as biomarkers of dry-cured ham processing has also been considered.
Keywords
UniProtNLCHClETDBSASDS–PAGEbovine serum albuminBasic Local Alignment Search ToolFormic acidhydrochloric acidsodium dodecyl sulphate polyacrylamide gel electrophoresisElectron transfer dissociationBlastBiomarkerZASPMass spectrometryuniversal protein resourceProteolysisPeptidesDry-cured hamhigh performance liquid chromatographyHPLC
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Marta Gallego, Leticia Mora, Paul D. Fraser, MarÃa-Concepción Aristoy, Fidel Toldrá,