Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7600701 | Food Chemistry | 2014 | 11 Pages |
Abstract
In Brazil, the consumption of extra-virgin olive oil (EVOO) is increasing annually, but there are no experimental studies concerning the phenolic compound contents of commercial EVOO. The aim of this work was to optimise the separation of 17 phenolic compounds already detected in EVOO. A Doehlert matrix experimental design was used, evaluating the effects of pH and electrolyte concentration. Resolution, runtime and migration time relative standard deviation values were evaluated. Derringer's desirability function was used to simultaneously optimise all 37 responses. The 17 peaks were separated in 19 min using a fused-silica capillary (50 μm internal diameter, 72 cm of effective length) with an extended light path and 101.3 mmol Lâ1 of boric acid electrolyte (pH 9.15, 30 kV). The method was validated and applied to 15 EVOO samples found in Brazilian supermarkets.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Cristiano Augusto Ballus, Adriana Dillenburg Meinhart, Francisco Alberto Jr., Roy Edward Bruns, Helena Teixeira Godoy,