Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7602244 | International Journal of Chemical and Analytical Science | 2013 | 5 Pages |
Abstract
This paper describes a reserved-phase HPLC method for detecting clenbuterol (CB) and ractopamine (RP) using an isocratic solvent-free mobile phase. The separations were performed a Kaseisorb® LC C1-300-5 (100 Ã 4.6 mm, 5 μm) with 0.05 mol/L octane sulfonic acid mobile phase and a photodiode array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. The detection limits were 0.68 ng/mL for CB and 1.24 ng/mL for RP, respectively. A harmless method for simultaneous detecting CB and RP was developed and may be further applied to the quantification in foods.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Naoto Furusawa,