Article ID Journal Published Year Pages File Type
7602290 International Journal of Chemical and Analytical Science 2013 5 Pages PDF
Abstract
A rapid, sensitive and specific method based on high performance liquid chromatography (HPLC) has been developed for the simultaneous determination of amitriptyline hydrochloride and Sildenafil citrate in the blood of rats. Sample preparation involved liquid-liquid extraction with n-hexane after alkalified with 0.5 M NaOH. Chromatographic separation was performed on XDB C-18 column with particle size of 5 μm (150 × 4.6 mm) with mobile phase acetonitrile and 20 mM KH2PO4 buffer, pH = 3 using flow rate 1.5 ml/min. Calibration curves were linear within the ranges of 0.25-50 and 0.1-50 μg/ml for amitriptyline hydrochloride and Sildenafil citrate, respectively. This method was successfully applied to the pharmacokinetic study in rats after oral administration of amitriptyline hydrochloride and Sildenafil citrate for 24 h.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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