Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7604248 | International Journal of Mass Spectrometry | 2015 | 7 Pages |
Abstract
- A novel method for the rapid formation of amide bonds in peptides within the mass spectrometer is described.
- Amino acids are prepared as anionic reagents with a ketenimine-activated N-terminus and a protected C-terminus.
- Gas-phase ion/ion reactions between the anionic reagents and doubly protonated “anchor” peptide cations result in extension of the “anchor” peptide with new amide bond formation at the C-terminus.
- This method does not use significant amounts of solvents and deprotecting agents that are required in the most current peptide synthesis methods.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhou Peng, Scott A. McLuckey,