Article ID Journal Published Year Pages File Type
7604248 International Journal of Mass Spectrometry 2015 7 Pages PDF
Abstract

- A novel method for the rapid formation of amide bonds in peptides within the mass spectrometer is described.
- Amino acids are prepared as anionic reagents with a ketenimine-activated N-terminus and a protected C-terminus.
- Gas-phase ion/ion reactions between the anionic reagents and doubly protonated “anchor” peptide cations result in extension of the “anchor” peptide with new amide bond formation at the C-terminus.
- This method does not use significant amounts of solvents and deprotecting agents that are required in the most current peptide synthesis methods.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
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