Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7605762 | International Journal of Mass Spectrometry | 2013 | 10 Pages |
Abstract
⺠Recombinant, mammalian-expressed prion protein contains a modification in the C-terminal domain. ⺠Lys193 is converted to hydroxylysine with a disaccharide added to the new hydroxyl. ⺠Mass spectrometry and Edman degradation demonstrate the presence of the modification. ⺠Molecular dynamics indicates that lys193 glycans produce stabilisation of a critical loop of PrP.
Keywords
PrPscO-linked glycosylationPTMPRNPPrPcGPiTSEMS/MScollisionally activated dissociationpost-translational modificationPost translational modificationtransmissible spongiform encephalopathyChoChinese Hamster OvaryCADMass spectrometryTandem mass spectrometryHydroxylysinePrion proteinrecombinant prion proteincellular prion proteinglycosylphosphatidylinositol
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mark A. Ritchie, Lawrence G. Hunt, Andrew C. Gill,