Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7606172 | International Journal of Mass Spectrometry | 2011 | 9 Pages |
Abstract
â¶ The structural dynamics and the Ca-induced conformational changes of the cardiac isoform of troponin are revealed by comparing H/D exchange rate constants for TnC alone, the binary TnC:TnI complex, and the ternary TnC:TnI:TnT complex for Ca-free and Ca-saturated states. â¶ TnC:TnI and TnC:TnI:TnT complexes possess both highly flexible and very rigid domains. â¶ The N-terminal extension of TnI (specific to the cardiac isoform), the DE linker in TnC alone, and the mobile domain of TnI exhibit fast H/D exchange rates. â¶ The present results corroborate prior X-ray crystallography and NMR interpretations and also illuminate domains that were absent or not resolved in those experiments.
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Authors
George M. Bou-Assaf, Jean E. Chamoun, Mark R. Emmett, Piotr G. Fajer, Alan G. Marshall,