| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7607352 | Journal of Chromatography A | 2018 | 7 Pages |
Abstract
A sensitive LC-MS/MS method was established to quantify diols and glycerol in fermentation broth using trichloroacetyl isocyanate as instantaneous derivatization reagent for monitoring the production of 1,3-propanediol and 2,3-butanediol from the biodiesel biorefinery process. Due to the derivatization reaction was very quickly at room temperature, only 1â¯min was needed for the reaction process. In addition, both extraction of analytes and evaporation of water were not employed in the analytical procedure. Furthermore, the isotope of chlorine was beneficial for understanding of the secondary mass spectrum and avoiding false positive results. Therefore, much more accurate results of diols and glycerol concentration in fermentation could be obtained even at very low levels for the evaluation of microbial metabolism pathway modification.
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Authors
Jun Chen, Dayu Chen, Xiaofan Zhang, Meng Wang, Biqiang Chen, Dongge An, Li Xu, Qiang Lyu,