Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7607370 | Journal of Chromatography A | 2018 | 11 Pages |
Abstract
A total of 48 chlorophylls and derivatives were identified and successfully determined in tea and processed vegetable and fruit foodstuff by UHPLC with photodiode-array and mass spectrometry detection. The method allowed the proper separation of chlorophyll derivatives resulting from demetallation, dephytilation, decarbomethoxylation, epimerisation and copperisation. The method was performed in less than 12â¯min, using an optimised ternary gradient (MeOH, iPrOH, MeCN and H2O with 10â¯mM of ammonium acetate) on an ACQUITY HSS T3 column. Mass spectrometry, applying both ESI and APCI ionization sources, was used for identification purposes. The method was applied to evaluate the degree of processing in teas of different origin and quality. It allowed differentiation between supermarket own-brand tea bags and teas sold by specialised shops. Pheophytins, pheophorbides and pyro derivatives were found mainly in processed green vegetable and fruit products thereof. However, chlorophyll-derived food colorants, such as Cu-chlorophyllins, Cu-pheophytins, Cu-pyropheophytins, Cu-pheophorbides and Cu-pyropheophorbides, were also detected in several products.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Antoni Delpino-Rius, Diana Cosovanu, Jordi Eras, Francesca Vilaró, Mercè Balcells, Ramon Canela-Garayoa,