Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7607505 | Journal of Chromatography A | 2018 | 23 Pages |
Abstract
Analysis of metabolites is often performed using separations coupled to mass spectrometry which is challenging due to their vast structural heterogeneity and variable charge states. Metabolites are often separated based on their class/functional group which in large part determine their acidity or basicity. This charge state dictates the ionization mode and efficiency of the molecule. To improve the sensitivity and expand the coverage of the mammalian metabolome, multifunctional derivatization with sheathless CE-ESI-MS was undertaken. In this work, amines, hydroxyls and carboxylates were labeled with tertiary amines tags. This derivatization was performed in under 100â¯min and resulted in high positive charge states for all analytes investigated. Amino acids and organic acids showed average limits of detection of 76â¯nM with good linearity of 0.96 and 10% RSD for peak area. Applying this metabolomic profiling system to bovine aortic endothelial cells showed changes in 15 metabolites after treatment with high glucose. The sample injection volume on-capillary was <300 cells for quantitative analyses. Targeted metabolites were found in human tissue, which indicates possible application of the system complex metabolome quantitation.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tianjiao Huang, Michael Armbruster, Richard Lee, Dawn S. Hui, James L. Edwards,