Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7607685 | Journal of Chromatography A | 2018 | 36 Pages |
Abstract
This study aims to develop a straightforward, sensitive UHPLC-MS/MS method to quantify 15 eicosanoids derived from arachidonic acid in human plasma. Tert-Butyl methyl ether was used on the liquid-liquid extraction method and significantly reduced the expense and time. The method showed excellent linearity for all analytes, with regression coefficients higher than 0.99 over a wide range of concentrations from 0.01â¯ngâ¯mLâ1 to 100â¯ngâ¯mLâ1. The recovery rates were over 65.00%, and the matrix effects ranged from 8.42% to 40.00%. The limits of detection ranged from 6â¯pgâ¯mLâ1 to 10â¯pgâ¯mLâ1, and all of the limits of quantification were 20 â 33â¯pgâ¯mLâ1. For the broad concentration range, the RE% for accuracy and precision were less thanâ¯Â±â¯15%. Moreover, trans-4-{4-[3-(4-Trifluoromethoxyphenyl)-ureido] cyclohexyloxy} benzoic acid (t-TUCB) pretreatment extended the window of detection for as much as 30â¯days. Eicosanoid signaling is altered in various neurological diseases, including pain, Alzheimer's disease and major depressive disorder. Therefore, this rapid, robust quantitative profiling of 15 eicosanoids in plasma could provide a distinct eicosanoid fingerprint for precision medicine in these patients.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yan Zhang, Meng-Yao Wang, Qi-Yuan Huang, Min-Zhen Zhu, Jing Ren, Xiong Cao, Wen-Chao Xiong, Xiao-Dan Xiao, Xin-Hong Zhu,