Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7607769 | Journal of Chromatography A | 2018 | 12 Pages |
Abstract
Mycotoxins are a group of secondary fungi metabolites present in foods that cause adverse effects in humans and animals. The objective of this study was to develop and validate a reliable and sensitive method to determine the presence of fumonisin B1, aflatoxin B1, ochratoxin B, T-2 toxin, ochratoxin A and zearalenone. A rapid, effective process, which involves microwave-assisted dispersive micro-solid phase extraction (MA-d-μ-SPE), has been proposed for the extraction and detection of 6 mycotoxins in peach seed, milk powder, corn flour and beer sample matrixes, for subsequent analysis by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). Several experimental parameters (type of dispersant, concentration of dispersant, vortex time, type of desorption solvent and pH) affecting the extraction efficiency were systematically studied and optimized. The optimum extraction conditions involved immersing 2.5â¯Î¼g/mL of nano zirconia (as dispersant) in a 5â¯mL sample solution. After 2â¯min of extraction by vigorous shaking, the target analytes were desorbed by 100â¯Î¼L of chloroform at pH 4.5. The results indicated good linearity in the range of 0.0074-3.6â¯Î¼g/mL (râ¯â¥â¯0.9982), low limits of detection (0.0036-0.033â¯Î¼g/kg for solid samples and 0.0022-0.017â¯ng/mL for beer), acceptable reproducibility (relative standard deviation (RSD%) 2.08-2.76% for retention time and 3.51-4.59% for peak area, nâ¯=â¯3), and satisfactory spiked recoveries (84.27-104.96%) for studied mycotoxins in sample matrixes, which demonstrated that MA-d-μ-SPE coupled with UHPLC-Q-TOF/MS is a useful tool for analysis of multi-mycotoxin.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Li-Jing Du, Chu Chu, Elisa Warner, Qiu-Yan Wang, Yu-Han Hu, Ke-Jie Chai, Jun Cao, Li-Qing Peng, Yu-Bo Chen, Juan Yang, Qi-Dong Zhang,