Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7607782 | Journal of Chromatography A | 2018 | 8 Pages |
Abstract
In the present study, we developed a simple and high-throughput solid phase extraction (SPE) procedure for selective extraction of catecholamines (CAs) in urine samples. The SPE adsorbents were electrospun composite fibers functionalized with 4-carboxybenzo-18-crown-6 ether modified XAD resin and polystyrene, which were packed into 96-well columns and used for high-throughput selective extraction of CAs in healthy human urine samples. Moreover, the extraction efficiency of packed-fiber SPE (PFSPE) was examined by high performance liquid chromatography coupled with fluorescence detector. The parameters affecting the extraction efficiency and impurity removal efficiency were optimized, and good linearity ranging from 0.5 to 400â¯ng/mL was obtained with a low limit of detection (LOD, 0.2-0.5â¯ng/mL) and a good repeatability (2.7%-3.7%, nâ¯=â¯6). The extraction recoveries of three CAs ranged from 70.5% to 119.5%. Furthermore, stable and reliable results obtained by the fluorescence detector were superior to those obtained by the electrochemical detector. Collectively, PFSPE coupled with 96-well columns was a simple, rapid, selective, high-throughput and cost-efficient method, and the proposed method could be applied in clinical chemistry.
Related Topics
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Authors
LiQin Chen, Hui Wang, Zhen Xu, QiuYue Zhang, Jia Liu, Jun Shen, WanQi Zhang,