Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7607938 | Journal of Chromatography A | 2018 | 14 Pages |
Abstract
The natural rubber molecule is one of the end products of isoprenoids metabolism in the plant. Dimethylallyl diphosphate (DMAPP) and farnesyl pyrophosphate (FPP) are two typical isoprenoids which control the rate of biosynthesis and the molecular weight of natural rubber. A rapid, nonradioactive method for quantitation of DMAPP and FPP in natural rubber latex by liquid chromatography tandem with mass spectrometry (LC-MS/MS) was reported. DMAPP and FPP were determined in the multiple reaction monitoring mode(MRM)followed by separation with a silica-based C18 column. The external standard quantitative method was established, and the results showed limits of quantitation (LOQs) were 28â¯ng/ml and 33â¯ng/ml for DMAPP and FPP, respectively. The concentrations were detected 70-96â¯ng/ml and 242-375â¯ng/ml for these two isoprenoids in natural rubber latex. Recoveries of the method were in the range 81-93%. Daytime comparison experiments found that FPP had better stability than DMAPP.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xi Zhang, Tianyang Guo, Tong Xiang, Yiyang Dong, Jichuan Zhang, Liqun Zhang,