Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608056 | Journal of Chromatography A | 2018 | 6 Pages |
Abstract
A rapid and sensitive PCR based strategy in combination with microchip capillary electrophoresis (MCE) was employed to simultaneously detect three foodborne pathogenic bacteria. Three pairs of primers were specially designed for the amplification of target genes from Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Salmonella enterica serovar Typhimurium (S. Typhimurium). The PCR products along with standard DNA fragments were employed to optimize the separation conditions in MCE. Under optimal conditions, detectable separation of the PCR products (1.6-3.5â¯ngâ¯Î¼Lâ1) from the three foodborne pathogenic bacteria was achieved within 135â¯s. The limits of detection of the three bacteria were concluded to be as low as 45â¯CFUâ¯mLâ1 for E. coli, 62â¯CFUâ¯mLâ1 for S. aureus and 42â¯CFUâ¯mLâ1 for S. Typhimurium. The RSD of migration time was in the range of 0.5-0.8%. We conclude that MCE along with PCR holds real potential for rapid analysis and detection of nucleic acids from routine foodborne pathogenic bacteria.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yan Zhang, Luqi Zhu, Yating Zhang, Pingang He, Qingjiang Wang,