Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608199 | Journal of Chromatography A | 2018 | 5 Pages |
Abstract
The recipes were applied in 5â¯Î¼m i.d.-capillaries with a length of 60â¯cm. Efficiencies under retained conditions amounted up to Nâ¯=â¯137,000 for the PLOT column with a layer thickness (df) of 300â¯nm and to Nâ¯=â¯109,000 for the PLOT column with dfâ¯=â¯550â¯nm. Working under conditions of similar retention, the narrow pore/high SA columns produced with the new 80â¯Â°C recipe generated the same number of theoretical plates as the wide pore size/low SA columns produced with the 95â¯Â°C recipe. This shows the 80â¯Â°C-hydrothermal treatment process allows for an increase in the phase ratio of the PLOT columns without affecting their intrinsic mass transfer properties and separation kinetics. This is further corroborated by the fact that the plate height curves generated with the new and former recipe can both be well-fitted with the Golay-Aris equation without having to change the intra-layer diffusion coefficient.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Takeshi Hara, Shunta Futagami, Wim De Malsche, Gino V. Baron, Gert Desmet,