Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608254 | Journal of Chromatography A | 2018 | 20 Pages |
Abstract
We synthesized Ni2+-attached O-Carboxymethyl chitosan Schiff base complexes embedded composite cryogels (Ni2+-O-CMCS-CCs) by means of polymerization of gel-forming precursors at subzero temperatures. Prepared affinity cryogel showed excellent adsorption performance for lysozyme selected as model protein to test adsorption parameters, demonstrating an adsorption capacity of 244.6â¯mg/g (15.3â¯mg/g for Ni2+ minus O-CMCS-CCs), with fast adsorption equilibrium within 30â¯min and good reversibility. The performance of Ni2+-O-CMCS-CCs for lysozyme was also evaluated by SDS-PAGE, and a purification efficiency of 86.9% with 89.5% purification yield was determined. The swelling test, FT-IR, and SEM analysis were carried out for the characterization of Ni2+-O-CMCS-CCs. At the end of 35 adsorption-desorption cycles, there was no significant change in the adsorption capacity.
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Physical Sciences and Engineering
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Authors
Ãmür Acet, Talat Baran, Demet Erdönmez, NeÅe Hayat Aksoy, İhsan Alacabey, Ayfer MenteÅ, Mehmet OdabaÅi,