Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608300 | Journal of Chromatography A | 2018 | 32 Pages |
Abstract
If all these figures of merit are considered, it is clear that this phenyl bonded wide-pore superficially porous stationary phase is one of the most promising materials to have been developed in recent years. Indeed, it offers kinetic performance comparable to the most efficient wide-pore SPP column on the market. In terms of protein recovery, this new phase was found to be superior to silica-based and silica-hybrid C4 bonded materials, particularly with separations performed at sub-80â¯Â°C temperature. Under such conditions, it in fact shows recoveries that are quite similar to a divinyl benzene (DVB) polymer-based material. More importantly, due to its unique, high coverage phenyl bonding, it offers additional steric effects and potentially even Ï-Ï interactions that yield advantageous selectivity for mAb sub-unit peaks and ADC species as compared to commonly used C4 or C18 bonded phases. Last but not least, mobile phases consisting of only 0.02-0.05% trifluoroacetic acid can be successfully used with this column, without significant loss in recovery and peak capacity.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Balázs Bobály, Matthew Lauber, Alain Beck, Davy Guillarme, Szabolcs Fekete,