| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7608553 | Journal of Chromatography A | 2018 | 10 Pages |
Abstract
Many polar columns with important structural hydrophobic moieties show dual retention mechanism, (Reversed Phase in water rich mobile phases and Hydrophilic Interaction at high acetonitrile concentrations). It is possible to select the mobile phase compositions in each of the two modes for separations of samples containing compounds largely differing in polarity. The three-parameter model describes the retention in each mode, with separately determined best-fit parameters. We applied the two-mode model to the retention data of sulfonamides and benzoic acid related compounds on a new polymethacrylate zwitterionic monolithic micro-column.
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Analytical Chemistry
Authors
Pavel Jandera, TomáÅ¡ Hájek, Zuzana Å romová,