Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608590 | Journal of Chromatography A | 2018 | 33 Pages |
Abstract
A robust and sensitive method of solid-phase extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established and performed for the simultaneous determination of eleven aminoglycosides (AGs) in royal jelly and honey. After sample extraction by a phosphate buffer containing trichloroacetic acid (TCA) and ethylenediaminetetracetic acid disodium salt (Na2EDTA), the extraction solution was subjected to a parallel solid-phase extraction for clean-up prior to the LC-MS/MS analysis. The same method was applied to analyze two completely different matrices, honey and royal jelly. Good sensitivity, repeatability, and recovery were obtained by using the mobile phase without an ion-pairing reagent such as heptafluorobutyric acid (HFBA) or sodium heptanesulfonate. The calibration curves of the honey and royal jelly samples exhibited a good linear response (R2â¯>â¯0.99) at six concentrations in the range of 10-1000â¯Î¼g/mL. The limit of quantification (LOQ) of the AGs ranged from 10 to 25â¯Î¼g/kg in the honey and from 12.5 to 25â¯Î¼g/kg in the royal jelly. The recoveries of the AGs for the honey and royal jelly samples were in the range of 79.48% to 108.95% and 74.61% to 113.70% respectively and the relative standard deviations (RSDs) were between 1.23% and 9.59%, and between 1.51% and 9.98%, respectively. The proposed approach has been allowed in China as a reference method for the simultaneous determination of eleven AGs in honey and royal jelly.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xinran Wang, Shupeng Yang, Yi Li, Jinzhen Zhang, Yue Jin, Wen Zhao, Yongxin Zhang, Jingping Huang, Peng Wang, Cuiling Wu, Jinhui Zhou,