Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608596 | Journal of Chromatography A | 2018 | 22 Pages |
Abstract
Advancement in high-throughput screening methods of novel therapeutic proteins for early stage research and development, specifically mAbs, have given mid-scale (milligram to gram scale) purification groups access to more of these molecules. The available purification technologies built to support mid-scale production was not efficient or versatile enough to keep up with this surge. To remedy this problem, we have designed and built a custom instrument using an ÃKTA Pure. This system enables parallel processing up to 5 samples and has the versatility to perform 1- to 3-step purification processes in a single queue. Furthermore, a unique purification scheme can be selected for each of the five samples in the queue. Overall processing time has reduced by 83% compared to manual, non-parallel load methods. Here, we describe our novel approach and demonstrate the flexibility, speed and efficiency of the instrument.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Darren E. Ferguson, Evan R. Mahan, Weijun Ma, Gabrielle Bitzas, Xiaotian Zhong, Richard Zollner, Aaron M. D'Antona,