Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608687 | Journal of Chromatography A | 2018 | 8 Pages |
Abstract
Prunus genus fruit seeds are sources of highly angiotensin-I-converting enzyme (ACE)âinhibitory peptides. The presence of peptides IYSPH, IYTPH, IFSPR, and VAIP seems to be related to this activity but no previous work has demonstrated the direct relationship between the concentration of these peptides and the antihypertensive activity of hydrolysates. This work describes the development of a method for the quantification of these peptides in Prunus seeds hydrolysates based on capillary liquid chromatographyâIT-MS/MS. The analytical characteristics of the method were evaluated through the study of the linearity, LOD, LOQ, presence of matrix interferences, precision, and recovery. The developed methodology was applied to the determination of the four peptides in seed hydrolysates from different Prunus genus fruits: peaches (7 varieties), plums (2 varieties), nectarines (3 varieties), apricots (2 varieties), cherry, and paraguayo. Peaches and plums seed hydrolysates yielded the highest concentrations of these peptides while paraguayo one showed the lowest concentrations. A high correlation between peptides concentrations was demonstrated suggesting that the four peptides could be released from the same seed proteins.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
EstefanÃa González-GarcÃa, MarÃa Concepción GarcÃa, MarÃa Luisa Marina,