Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7608798 | Journal of Chromatography A | 2018 | 4 Pages |
Abstract
The separation and detection of these stereoisomers was achieved using a Chiralcel OD-H, 4.6â¯Ãâ¯250â¯mm, 5â¯Î¼m dp column with heptane: ethanol: dichloromethane in a ratio of 95:3:2 (v:v:v) as mobile phase at a flow rate of 0.7â¯mL/min. UV detection was carried out at 245â¯nm and the column temperature was maintained at 15â¯Â°C. The analytical method was phase appropriately validated. The limit of detection and limit of quantification were found to be 0.035 and 0.07â¯Î¼g, respectively. The newly developed method has been implemented for routine utilization to monitor the chiral control during process development and used as the quality control method for chiral purity of the desired compound.
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Analytical Chemistry
Authors
Nilesh Joshi, Balaji Dhamarlapati, Athimoolam Pillai, Justine Paulose, Jessica Tan, Laura E. Blue, Jason Tedrow, Bob Farrell,