Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7609213 | Journal of Chromatography A | 2018 | 22 Pages |
Abstract
A novel screening and quantitation method is reported for non-target multiresidue analysis of pesticides using ultra-HPLC-quadrupole-Orbitrap mass spectrometry in spice matrices, including black pepper, cardamom, chili, coriander, cumin, and turmeric. The method involved sequential full-scan (resolutionâ=â70,000), and variable data independent acquisition (vDIA) with nine consecutive fragmentation events (resolutionâ=â17,500). Samples were extracted by the QuEChERS method. The introduction of an SPE-based clean-up step through hydrophilic-lipophilic-balance (HLB) cartridges proved advantageous in minimizing the false negatives. For coriander, cumin, chili, and cardamom, the screening detection limit was largely at 2âng/g, while it was 5âng/g for black pepper, and turmeric. When the method was quantitatively validated for 199 pesticides, the limit of quantification (LOQ) was mostly at 10âng/g (excluding black pepper, and turmeric with LOQâ=â20âng/g) with recoveries within 70-120%, and precision-RSDs <20%. Furthermore, the method allowed the identification of suspected non-target analytes through retrospective search of the accurate mass of the compound-specific precursor and product ions. Compared to LC-MS/MS, the quantitative performance of this Orbitrap-MS method had agreements in residue values between 78-100%.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Arnab Goon, Zareen Khan, Dasharath Oulkar, Raviraj Shinde, Suresh Gaikwad, Kaushik Banerjee,