Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7609406 | Journal of Chromatography A | 2017 | 6 Pages |
Abstract
Specific monoclonal antibodies were coupled with magnetic Sepharose-based beads and used, for the first time. The methodology was applied to preconcentrate anatoxin-a from water and the later determination by ion mobility spectrometry (IMS). Dispersive magnetic immunoaffinity (d-MagIA) extraction methodology provided a limit of detection of 0.02 μg Lâ1 and a satisfactory precision with a relative standard deviation lower than 15%. Recoveries were evaluated at 0.5, 1.0 and 5.0 μg Lâ1 anatoxin-a with quantitative values from 91 to 115%. Additionally, isobaric interferences with phenylalanine were completely avoided by the use of the developed d-MagIA extraction coupled to IMS determinations.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tinh Le, Francesc A. Esteve-Turrillas, Sergio Armenta, Miguel de la Guardia, Guillermo Quiñones-Reyes, Antonio Abad-Fuentes, Antonio Abad-Somovilla,