Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7609537 | Journal of Chromatography A | 2017 | 9 Pages |
Abstract
In this study, a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method, optimized by a 23 full factorial design, was developed for the determination of 72 pesticides in plant parts of carrot, corn, melon, rice, soy, silage, tobacco, cassava, lettuce and wheat by ultra-high-performance liquid chromatographic tandem mass spectrometry (UHPLC-MS/MS). Considering the complexity of these matrices and the need of use calibration in matrix, a new calibration approach based on single level standard addition in the sample (SLSAS) was proposed in this work and compared with the matrix-matched calibration (MMC), the procedural standard calibration (PSC) and the diluted standard addition calibration (DSAC). All approaches presented satisfactory validation parameters with recoveries from 70 to 120% and relative standard deviations â¤Â 20%. SLSAS was the most practical from the evaluated approaches and proved to be an effective way of calibration. Method limit of detection were between 4.8 and 48 μg kgâ1 and limit of quantification were from 16 to 160 μg kgâ1. Method application to different kinds of plants found residues of 20 pesticides that were quantified with z-scores values â¤Â 2 in comparison with other calibration approaches. The proposed QuEChERS method combined with UHPLC-MS/MS analysis and using an easy and effective calibration procedure presented satisfactory results for pesticide residues determination in different crop plants and is a good alternative for routine analysis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mariela S. Viera, Tiele M. Rizzetti, Maiara P. de Souza, Manoel L. Martins, Osmar D. Prestes, Martha B. Adaime, Renato Zanella,