Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7609816 | Journal of Chromatography A | 2017 | 11 Pages |
Abstract
Experimental van Deemter curves indicate that the reduced velocity in the precolumn at 250 μL/min flow rate is about 50. Nonetheless, about 1000 theoretical plates are generated. When operating as a precolumn, clear advantages are seen for solutes across a range of modest k' values (2.2-23.4 at the separation conditions at 65 °C) using TASF alone (5 °C) with 50 μL injection volumes of methyl through n-butyl parabens, and with 100 μL injections that also include solvent-based focusing (90:10 aqueous/acetonitrile sample, 80:20 mobile phase).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Stephen R. Groskreutz, Anthony R. Horner, Stephen G. Weber,