| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7609922 | Journal of Chromatography A | 2016 | 47 Pages |
Abstract
The present publication describes a novel platform for purifying Expi293Fâ¢-expressed recombinant mAbs directly from cell-free culture supernatant on a Perkin Elmer JANUS-VariSpan ALH equipped with a plate shuttle device. The purification platform allows automated 2-step purification (Protein A-desalting/size exclusion chromatography) of several hundred mAbs per week. The new robotic method can purify mAbs with high recovery (>90%) at sub-milligram level with yields of up to 2 mg from 4 mL of cell-free culture supernatant.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Peter M. Schmidt, Michael Abdo, Rebecca E. Butcher, Min-Yin Yap, Pierre D. Scotney, Melanie L. Ramunno, Genevieve Martin-Roussety, Catherine Owczarek, Matthew P. Hardy, Chao-Guang Chen, Louis J. Fabri,